The Study

Best Practices — Herbs and Spices: Powerful in Small Amounts, Precise Measurement Needed

Spices are at their most obvious as taste enhancers.

No where is this more clearly illustrated than the results of a study that every mother would rank as a near-miracle — spices can induce high school students to like vegetables.

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At the foundational level, herbs and spices are well-tested additives used in small concentrations that not only can help food taste good, but which have also played beneficial roles in human evolution.

This is important to recognize because “additives” used in food processing have become a b??te noir among nutritionists which point toward modern additives in “ultra processed” foods as contributors to the current epidemics in obesity and Type 2 Diabetes (T2D)

It is worthwhile to note that dietary intervention studies investigating ultra-processed vs. minimally processed foods have neglected to devote sufficient attention and proper quantification to the significant effects of herbs and spices which extend beyond sensory effects. This lack of attention and precision has contributed to replication issues and to the lack of specific causal data connecting modern additives to intervention study outcomes and conclusions.

Herbs and spices: Taste good to be good?

Prime examples of the survival benefits offerted by herbs and spices this can be found in this expansive Bioscience journal article by Sherman and Billing: “Darwinian Gastronomy: Why We Use Spices: Spices taste good because they are good for us.”

The article’s broad survey of the various health and taste properties of spices, includes ample supporting data, including the anti-bacterial properties that code for survival of the species and, thus, have affected human evolution.

One eye-opening example from that article is a chart showing how pepper, chilis, coriander, cumin as well as garlic and onions — all known for anti-bacterial effects, especially in meat — are most common in equatorial latitudes and steadily decrease towards the poles.

Right-click image to view a larger version.

Culinary Darwinism

To recapitulate the concept described by Sherman and Billing: Those people who found the taste of herbs and spices pleasant were protected from the illnesses of bacterial infections. They survived, procreated, and spread a legacy of protected gastronomy.

Those who did not like the taste of herbs and spices ate food without bacterial protection and were ejected from the gene pool.

The same mechanism holds true for herbs and spices that exhibit beneficial effects on digestion, metabolism, controlling inflammation and other biological processes.

Further, if the current increases in obesity and T2D can be definitively and causally defined as resulting from modern additives, then we are seeing another phase of evolutionary selection for survival characteristics.

Spices: metabolism, digestion, and hormones

In addition to playing roles as appetite enhancers, preservatives and antibacterial agents, spices play significant direct roles in regulating metabolism, energy balance, appetite regulatory hormones and can influence inflammation and insulin resistance.

See links at the bottom of this post for a sampling of the many sources in this area.

Because those non-taste factors also affect the amount that a test subject eats, and exert other physiological effects that can influence the outcome of an intervention study, spice levels must be tightly controlled and measured precisely,

Equal spice levels in identical menu items in both legs of an intervention study are vital for replicable results

Measuring herbs and spices precisely is particularly vital in dietary studies that rely on biological reaction outcomes such as C-Reactive Protein, Ghrelin, insulin resistance

To insure that a study can be replicated, investigators should assure that spice levels are the same in meals in both legs. Otherwise, failure to serve the exact amount of each spice in both legs of an intervention will affect the amounts consumed as well as biological and clinical indicators.

This is especially important in ad libitum studies, but can also play a role when test subjects find the food in one leg or the other unpalatable and do n0t consume the entire meal.

Palatability will obviously affect the amount of food consumed. And this will, in turn, affect the amount of weight gained or loss by ad libitum study subjects.

Spices: big impact in small quantities means precise measurement required

While the ingredients in cooked dishes are measured in tens or hundreds of grams per portion, herbs and spices are usually called for in fractions of a gram.

As a result, measurements need to be precise and that calls for measurement by weight rather than volume.

Right-click image to view a larger version.

Right-click image to view a larger version.

For example, recipes may call for a teaspoon of salt, a half teaspoon of ground cinnamon or a tablespoon of dried ground basil. The actual mass of those volumes can vary depending upon the moisture content, the granularity of the substance, how densely (or lightly) compacted, is it an exact level measure. But a gram (or half gram) will make for a more precise measure.

Best Practice: All measurements of spices and other foods must be measured by weight, not volume. Volumetric measurements involve many opportunities for error: density and packing of dry ingredients will affect volume. So to, errors in the reading of meniscus levels, accidental over- or under-pouring and the lack of precision or ability to calibrate culinary apparatus. With very minor adjustments, measurements by weight adapt easily to the activity and need for efficiency of a kitchen

Best Practice: Avoid plastic contact. Leaching and microplastic contamination are ubiquitous.

Pepper mill manufactured with all glass, stainless steel and ceramic grinder parts. – Right-click image to view a larger version.

The pepper mill’s ceramic grinder parts – Right-click image to view a larger version.







See also: Best practices: Scale calibration

Sources: BPA Spice and food concentrations

Unexpected results in a randomized dietary trial to reduce phthalate and bisphenol A exposures Sathyanarayana, S., Alcedo, G., Saelens, B. et al. Unexpected results in a randomized dietary trial to reduce phthalate and bisphenol A exposures. J Expo Sci Environ Epidemiol 23, 378???384 (2013).

Concentrations of bisphenol A in the composite food samples from the 2008 Canadian total diet study in Quebec City and dietary intake estimates X.-L. Cao, C. Perez-Locas, G. Dufresne, G. Clement, S. Popovic, F. Beraldin, R.W. Dabeka & M. Feeley (2011) Concentrations of bisphenol A in the composite food samples from the 2008 Canadian total diet study in Quebec City and dietary intake estimates, Food Additives & Contaminants: Part A, 28:6, 791-798, DOI: 10.1080/19440049.2010.513015

Bisphenol A: Food Exposure and Impact on Human Health Almeida, S., Raposo, A., Almeida???Gonz??lez, M. and Carrascosa, C. (2018), Bisphenol A: Food Exposure and Impact on Human Health. Comprehensive Reviews in Food Science and Food Safety, 17: 1503-1517. doi:10.1111/1541-4337.12388

Physiological effects of spices

Darwinian Gastronomy: Why We Use Spices: Spices taste good because they are good for us – Paul W. Sherman, Jennifer Billing, Darwinian Gastronomy: Why We Use Spices: Spices taste good because they are good for us, BioScience, Volume 49, Issue 6, June 1999, Pages 453???463,

Spices and energy balance

The influence of herbs and spices on overall liking of reduced fat food

Effects of Two Doses of Curry Prepared with Mixed Spices on Postprandial Ghrelin and Subjective Appetite Responses???A Randomized Controlled Crossover Trial

Polyphenol-rich spice-based beverages modulated postprandial early glycaemia, appetite and PYY after breakfast challenge in healthy subjects: A randomized, single blind, crossover study

Google Scholar search: spices and appetite

Herbs and spices increase liking and preference for vegetables among rural high school students

A Spoonful of Lead
A 10-Year Look at Spices as a Potential Source of Lead Exposure

Ground Turmeric as a Source of Lead Exposure in the United States

Polyphenol-rich curry made with mixed spices and vegetables increases postprandial plasma GLP-1 concentration in a dose-dependent manner

Google Scholar Search: turmeric anti inflammatory

Google Scholar Search: spices influence appetite

FDA Import Alert 28-13: Lead in Turmeric

Stanford study describes spice makers adding lead to turmeric

Turmeric means ???yellow??? in Bengali: Lead chromate pigments added to turmeric threaten public health across Bangladesh